Publié le

Femoral Arterial and Venous Catheterization

JOVE Free Video
For Blood Sampling, Drug Administration and Conscious Blood Pressure and Heart Rate Measurements
Jespersen, B., Knupp, L., Northcott, C. A. Femoral Arterial and Venous Catheterization for Blood Sampling, Drug Administration and Conscious Blood Pressure and Heart Rate Measurements. J. Vis. Exp. (59), e3496, doi:10.3791/3496 (2012).

Chronic catheterization of blood vessels in the rat is often required for administration of substances, obtain blood sample over a period of time or for direct conscious blood pressure measurements. Femoral arterial catheterization of the rat and corresponding measurements of blood pressure in the conscious animal will be demonstrated.

Date Published: 1/24/2012, Issue 59; doi: 10.3791/3496

Keywords: Medicine, Issue 59, Rat, catheter, blood pressure, vein, artery, blood sampling, surgery, femoral

Publié le

iPRECIO™ – Journal of Pharmacological and Toxicological Methods

Original article

A novel type of implantable and programmable infusion pump for small laboratory animals

Chikara Abe, Takayuki Tashiro, Kunihiko Tanaka, Ryousuke Ogihara, Hironobu Morita


Introduction: The iPRECIO™ (Primetech Corporation, Tokyo, Japan) is a new form of pump for infusing small laboratory animals. The key features of the iPRECIO™ are that it can be implanted within the animal, it is refillable, and it is programmable. The infusion start-points and end-points are adjustable, infusion rate can be altered, and the infusion solution can be changed after the pump is implanted. In order to confirm the precision of the iPRECIO™, in vivo and in vitro experiments were employed.

Methods: In the in vitro experiment, at the excretion rate of 1 μl/h for 336 h, 15 μl/h for 96 h, and 30 μl/h for 120 h, the decrease in each pump weight was used to estimate the actual excretion volume. In the in vivo experiments, the iPRECIO™ was chronically implanted in rats, angiotensin II was infused, and arterial pressure (AP) was monitored. Results: In the in vitro experiment, the volume of solution excreted from the pump increased with time, and the volume excreted matched the programmed volume. The infusion rate also changed at the scheduled time. In the in vivo experiment, AP increased and decreased on schedule, and a dose-dependent pressor response to angiotensin II occurred. Furthermore, after exchanging saline with angiotensin II, AP increased and decreased on schedule.

Discussion: Present data of the in vitro and in vivo experiments indicates that the iPRECIO™ worked precisely, making it suitable for a variety of experiments involving small laboratory animals.
© 2008 Elsevier Inc. All rights reserved.

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